Mouse ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit
SKU: 67156459169

Mouse ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit

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Description

Mouse ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA KitWith about 17 known citations, the Mouse ICAM 1 CD54 (intercellular adhesion molecule 1) ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse ICAM 1 CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM 1 CD54 and Avidin Horseradish Peroxidase

With about 17 known citations, the Mouse ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ICAM-1/CD54. You can calculate the concentration of Mouse ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

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SKU: 67156459169

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4.4 ★★★★★
Based on 1015 reviews
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Verified Purchase
Bernard Eisenfeld, M.D.
Phoenix, US
★★★★★ 5
This is the one.
Size: 58.3mm, Color: Black
As an el Supremo Coffee Geek, I've wasted money on lots of different tampers. Save your money and buy this one or it's equivalent. This thing is heavy duty, solid, well-made, self-leveling, tamps at 30 pounds and is fool-proof even for fools. Also, it's a palm tamper meaning more fun and more satisfying to use as you prep the puck for your morning buzz. Buy it. You will thank me.
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Reviewed in the United States on March 30, 2024
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lesserof2weevils
Grantham, US
★★★★★ 5
Super easy to use and takes the guess-work out of getting even pressure on the grounds
Size: 58.3mm, Color: Black, Size: 58.3mm, Color: Black
I have been using a hand tamper for many years and have a good idea of the pressure I am applying to the grounds in the portafilter. I honestly considered spring tampers to be a bit silly. But this tamper really does take the guess-work out of pressing the coffee grounds. The shroud helps guide the press so that pressure is applied even and flat across the surface. With the manual tamper, there will be some play when pressing, or some unevenness in pressure. This is especially true when cranking out many espressos during a breakfast party with friends. Having the spring tamper makes quick work of turning out a lot of coffees with consistency. There are no instructions for taking this tamper apart to clean inside, and you may not really need to do that very often. After each use, just pull back the shroud (the black piece that moves up and down) and wipe off the tamper (the shiny steel part). This will help prevent grounds from working their way inside. If you do want to take it apart, it’s easy. Pull back the shroud and unscrew the tamper. It will come apart into three pieces. Wipe the pieces off and reassemble. Super quick and easy. This tamper is very heavy. My scale shows 656 g (about a pound and a half). It fits the portafilter exactly, with no slop. Just place on top of grounds and press!. Don’t even worry about pressure, just push down all the way. Overall I had fun using this tamper versus my manual one. And since I make myself a cappuccino very early in the morning, I don’t have to be very awake to get a good pack on the grounds and get my coffee fast!
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Reviewed in the United States on January 10, 2024
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Verified Purchase
Mike Steven
Lake Worth, US
★★★★★ 5
Quality and Function
Looks like a silly little thing, I am not a coffee enthusiast. But it does exactly what it is supposed to do and with high quality. The device (a stretch to call it that) is made of sturdy materials including the needles. They give you two different thicknesses of needles, one for expressi (0.3) and one set for coffee grinds (0.4). Each comes wth some spares. The stand is well designed and keeps grinds from spreading all over. It is very elegant design. I use this with a dosing funnel on my portafilter. Not the cheapest one could buy but well worth the money.
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Reviewed in the United States on March 16, 2025
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Verified Purchase
Sunny
Boise, US
★★★★★ 5
Well made, high quality product.
This product demonstrates excellent quality, featuring a robust construction and intuitive design for ease of use. It includes two distinct wire sizes and offers straightforward assembly. Its superior durability and strong magnetic properties ensure effortless reattachment to its stand.
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Reviewed in the United States on June 30, 2025
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Verified Purchase
Amazon Customer
Waukegan, US
★★★★★ 5
Perfect WDT tool
Best WDT I’ve found. Love that it includes 0.3mm needles (as I’ve found that 0.4mm needles push the grounds around too much), and the stand is very clever. It knocks off the residual grounds on the needles and is easy to use. As far as WDT tools go, I can’t imagine anything better.
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Reviewed in the United States on April 13, 2025

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